User manual BIOPTECHS FCS3

[. . . ] Place a microaqueduct slide, groove side up, onto the gasket and oriented such that the perfusion tubes align with the holes in the microaqueduct slide. Select an upper gasket of the appropriate thickness and internal geometry for your experiment on the microaqeduct slide and press in place. Place the PEEK pressure plate on top of the coverslip (wide edge down) oriented in a manner to engage the two recesses on the perimeter of the base. Screw the knurled chamber top onto the dovetailed chamber base thus compressing the gaskets to the optical components. [. . . ] To adjust the setpoint set the numeric display to Setpoint, then adjust the potentiometer to the desired value. If you need to change the setpoint value during your experiment on a routine basis there is a remote setpoint jack on the back of the controller. Slide is the location where the calibration of the circuit reading the Microaqueduct Slide can be adjusted. Chamber is the location where the calibration of the circuit reading the Chamber can be adjusted. Calibrate substitutes precision resistors into the circuit that reads the thermistors so that calibration adjustments can be made to a known value. 3560 Beck Road · Butler, PA 16002 · Voice: 724-282-7145 · Fax: 724-282-0745 · E-mail: info@bioptechs. com · Web: www. bioptechs. com Chamber is a six pin mini DIN socket where the FCS3 is connected. Back Panel of the FCS2 & FCS3 Controller AC Power line input, power switch, voltage selector, and fuses are all incorporated in the AC Power Input Module. The controller can operate between 90 - 260V 50-60Hz, or a 24V battery can be used as an alternate power supply. The fuses can be accessed by prying the fuse block out of its socket with a small screwdriver wedged under the small slot in the opening of the AC line receptacle. When replacing the fuse block, make sure to align the arrow on the outside of the fuse block with the closest voltage value to your supply line voltage: For 90-130 supply line voltage place the fuse block with the arrow pointing to 110-120V. For 180-260 supply line voltage place the fuse block with the arrow pointing to 220-240V. Temp Out is a voltage output direct from two instrument amplifiers reading the thermistors from channel A and B. The left pin (as you look at the back of the controller) is channel A, center pin is channel B, and right pin is ground. Remote Setpoint is an analog input that allows the user to set the controllers setpoint from an external DC source. A DC voltage can be applied to the tip of this jack equal to temperature /10. When the jack is inserted into the controller it will automatically switch to the jacks value. When removed it will default to the setting on the front of the controller. Use for programming or cycling operating temperatures. 3560 Beck Road · Butler, PA 16002 · Voice: 724-282-7145 · Fax: 724-282-0745 · E-mail: info@bioptechs. com · Web: www. bioptechs. com Controller Operation: 1. Turn the unit on using the main on-off switch located on the rear panel. Insert the enclosed screwdriver into the hole marked Setpoint and adjust the controller to the desired setpoint. Set the display to the "Slide" or "Chamber" position. [. . . ] Therefore, there is no need for an alarm on the chamber frame circuit. · · The display can be switched between Set, Slide and Chamber at any time to monitor temperature without interfering with the regulation. If the Chamber was preheated it will come up to temperature in about 1 minute. Note: The vertical portion of the drain tubing, which extends down from the stage to your waste receptacle will create a siphon and form negative pressure in the chamber. This can be eliminated by breaking the siphon at a point equal to the height of the specimen with a "T" fitting. Continual Perfusion with the Micro-Perfusion Pump: Bioptechs recommends the FCS3 Micro-Perfusion Pump due to its smooth flow profile in slow, flow applications. [. . . ]